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human genome oligo array (22 k)  (CapitalBio Corporation)

 
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    CapitalBio Corporation human genome oligo array (22 k)
    AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). <t>The</t> <t>CapitalBio</t> Human Genome <t>Oligo</t> Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.
    Human Genome Oligo Array (22 K), supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human genome oligo array (22 k)/product/CapitalBio Corporation
    Average 90 stars, based on 1 article reviews
    human genome oligo array (22 k) - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Isolation and characterization of adult mammary stem cells from breast cancer-adjacent tissues"

    Article Title: Isolation and characterization of adult mammary stem cells from breast cancer-adjacent tissues

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.6485

    AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). The CapitalBio Human Genome Oligo Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.
    Figure Legend Snippet: AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). The CapitalBio Human Genome Oligo Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.

    Techniques Used: Gene Expression, Hybridization, Labeling, Modification, Reverse Transcription Polymerase Chain Reaction, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Western Blot, Polymerase Chain Reaction



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    AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). <t>The</t> <t>CapitalBio</t> Human Genome <t>Oligo</t> Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.
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    Image Search Results


    AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). The CapitalBio Human Genome Oligo Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.

    Journal: Oncology Letters

    Article Title: Isolation and characterization of adult mammary stem cells from breast cancer-adjacent tissues

    doi: 10.3892/ol.2017.6485

    Figure Lengend Snippet: AMSCs exhibit differential gene expression compared with autologous breast cancer cells. (A) Representative image of four blocks following the hybridization of Cy3-labeled AMSC sample (green signal) and Cy5-labeled breast cancer sample (red signal). The CapitalBio Human Genome Oligo Array (22K) includes 21,522 70-mer oligo probes spotted on a 75×25-mm, chemically modified glass slide. The green color indicates a stronger signal from the AMSC sample, whereas red indicates a stronger signal from the breast cancer sample, and yellow indicates a similar signal from the two tissue types. (B) Gel electrophoretic analyses of RT-PCR products. Total RNA was extracted from isolated breast cancer cell samples and AMSC samples selected at random from the 9 patients with breast cancer, and was reverse transcribed. The relative gene expression levels of MYC, RAS, and ERBB2 were examined by semi-quantitative PCR, with ACTB used as an internal control. (C) Western blot analysis of Ras, ErbB2 and PTEN in breast cancer cell samples and AMSC samples with β-actin used as internal control. AMSC, adult mammary stem cell; RT-PCR, reverse transcription-polymerase chain reaction; ErbB2, ErbB receptor tyrosine kinase 2; ACTB, β-actin; PTEN, phosphatase and tensin homolog.

    Article Snippet: The gene expression profiles of the isolated AMSCs and breast cancer cells of the 9 patients were determined using a CapitalBio Human Genome Oligo Array (22 K) (cat. no. 400010; CapitalBio Corporation, Beijing, China) of 21,552 human genes, according to the manufacturer's instructions.

    Techniques: Gene Expression, Hybridization, Labeling, Modification, Reverse Transcription Polymerase Chain Reaction, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Western Blot, Polymerase Chain Reaction